Diffusion of lactic acid in cell-seeded tissue engineering scaffolds
While many studies in the literature assumed a similar value for the glucose diffusivity in both water and cell culture media (CCM), Suhaimi et al hypothesized the difference in the composition and hydrodynamic properties of both media should give different respective diffusivities. The results shown have proven the hypothesis to be correct. The glucose diffusivity in CCM has been found to be significantly reduced than the one in water due to CCM having a larger dynamic viscosity than water. Another reason may be due to the presence of extra components and therefore the difference in fluid properties of CCM. Although the result from this research does not exclusively apply to all other biological media/cultures since the variation in composition of media may imply a different diffusivity value, it does highlight the danger of assuming glucose diffusivity in CCM as equal to that in water. Similar to the self-diffusivity of glucose in CCM, the effective diffusivity for tissue engineering (TE) materials imbibed in CCM has also been found to be significantly smaller than those in water which is contrary to what have been generally assumed in the previous studies. This further proves that the presence of extra components is a contributing factor to a difference in the effective diffusivity value. Lactic acid is an example of a metabolic waste product produced by cells. As similar to the vascular system in vivo, we should mimic the system in such a way that the diffusion of lactate acid within the scaffold is also monitored. In this way, it may complete the biochemical communication and especially useful in developing a mathematical model that can simulate real situations.
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