Dr Mark I.R. Petalcorin

Protein Scientist/Molecular Biologist/Biochemist with graduate degree ( PhD in Molecular Biology, MSc in Biochemistry) from Osaka University and PhD thesis on investigating chloride Ion channels using model systems

• 5-year full time undergraduate teaching experience at University of the Philippines
• 3-year experience as a Postdoc in a Pharmaceutical research unit at Kings College London
Working on drug metabolizing protein, flavin monooxygenase ( FMO)
• A further 10-year experience in a Senior research scientific post at London Research Institute,
Cancer Research UK working on proteins involved in DNA damage response/repair pathways focusing on roles of helicases, recombinases and polymerases in homologous recombination after DNA double-strand breaks
• Expert in large-scale protein identification/interaction and quantification using liquid chromatography/mass spectrometry (LC-MS, MALDI-TOF)
• Expert in recombinant protein expression and purification using various host organisms from bacteria, insect to human cells
• Expert in conducting in-vitro biochemical assays to analyze protein-protein and protein-DNA interactions and in-vivo cell imaging to analyze protein activities, localization and dynamics
• An accomplished analytical scientist with knowledge, skills and adaptability of employing new systems and recent technologies including the use of protein/DNA analyzers, mass spectrometers, HPLC, DHPLC, FPLC ( AKTA purifier Prime, Explorer), micromanipulators and microinjectors (FemtoJet and Narashige ), particle bombardment apparatus, confocal laser microscopes for live cell imaging and the utility of softwares for data acquisition and analysis
• Expert in genome engineering/ transgenesis to modify genomes of model organisms and mammalian/human cells for cancer and disease studies
• More than 16 papers and publications in high impact journals including Nature, Cell, Science, Molecular Cell, Genes Dev., PNAS, Journal of Molecular Biology and Plos Genetics
• Excellent computer skills in using DNA, protein and database analysis softwares ( Xcalibur, Mascot, Scaffold, Perseus, MaxQuant, MacVector, VectorNTI, Lasergene DNAStar, Unicorn, Fluoview FV1000 etc.)

RESEARCH INTERESTS

Research interests in Cancer proteomics (mass spectrometry and peptide arrays), biochemistry (in vitro protein-protein, protein-DNA or protein-substrate binding assays and analysis, structure prediction studies) and genetics (model organism C. elegans breeding and outcrossing) focusing on genome stability, meiosis, homologous recombination and DNA repair pathways.

RECENT PUBLICATIONS

Chung G, Rose AM, Petalcorin MI, Martin JS, Kessler Z, Sanchez-Pulido L, Ponting CP, Yanowitz JL and SJ Boulton (2015) REC-1 and HIM-5 distribute meiotic crossovers and function redundantly in meiotic double-strand break formation in Caenorhabditis elegans. Genes Dev. 29(18): 1969-79.

Ceccaldi R, Liu J, Amunugama R, Hajdu I, Primack B, Petalcorin MI, O’Connor KW, Konstantinopoulos PA, Elledge SJ, Boulton SJ, Yusufzai T and AD D’Andrea (2015) Homologous recombination (HR) - deficient tumors are hyperdependent on POLQ- mediated repair. Nature 518(7538): 258-62.

Frizzell A, Nguyen J, Petalcorin MI, Turner KD, Boulton SJ, Freudenreich CH and RS Lahue (2014) RTEL1 inhibits trinucleotide repeat expansions and fragility. Cell Reports 6(5): 827-835.

Vannier JB, Sandhu S, Petalcorin MI, Wu X, Nabi Z, Ding H, and SJ Boulton (2013) RTEL1 is a replisome associated helicase that promotes telomere and genome-wide replication. Science 342: 239-242.

Vannier JB, Pavicic-Kaltenbrunner V, Petalcorin MI, Ding H and SJ Boulton (2012) RTEL1 dismantles T loops and counteracts telomeric G4-DNA to maintain telomere integrity. Cell 149(4): 795-806.

TOP PUBLICATIONS

Ceccaldi R, Liu J, Amunugama R, Hajdu I, Primack B, Petalcorin MI, O’Connor KW, Konstantinopoulos PA, Elledge SJ, Boulton SJ, Yusufzai T and AD D’Andrea (2015) Homologous recombination (HR) - deficient tumors are hyperdependent on POLQ- mediated repair. Nature 518(7538): 258-62.

Vannier JB, Sandhu S, Petalcorin MI, Wu X, Nabi Z, Ding H, and SJ Boulton (2013) RTEL1 is a replisome associated helicase that promotes telomere and genome-wide replication. Science 342: 239-242.

Vannier JB, Sandhu S, Petalcorin MI, Wu X, Nabi Z, Ding H, and SJ Boulton (2013) RTEL1 is a replisome associated helicase that promotes telomere and genome-wide replication. Science 342: 239-242.

Ward JD *, Muzzini DM *, Petalcorin MI*, Martinez-Perez E, Martin JS, Plevani P, Cassata G, Marini F, SJ Boulton (2010) Overlapping mechanisms promote postsynaptic RAD-51 filament disassembly during meiotic double-strand break repair. Molecular Cell 37:259-72. *joint co-first authors

Petalcorin MI, Galkin VE, Yu X, EH Egelman and SJ Boulton (2007) Stabilization of RAD-51- DNA filaments via an interaction domain in Caenorhabditis elegans BRCA2. Proc Natl Acad Sci USA. 104:8299-304.

GRANT DETAILS

URG - URG32
Biochemical characterization of REC-1 and its interacting partners
Dr Mark I.R. Petalcorin(PI), Dr Adi Idris(Co-PI), Dr Jose H. Santos(Co-PI)
BND 20000
01/10/2015 - 30/09/2016